MiR-10a in Pancreatic Juice as a Biomarker for Invasive Intraductal Papillary Mucinous Neoplasm by miRNA Sequencing

New biomarkers are needed to further stratify the risk of malignancy in intraductal papillary mucinous neoplasm (IPMN). Although microRNAs (miRNAs) are expected to be stable biomarkers, they can vary owing to a lack of definite internal controls. To identify universal biomarkers for invasive IPMN, we performed miRNA sequencing using tumor-normal paired samples. A total of 19 resected tissues and 13 pancreatic juice samples from 32 IPMN patients were analyzed for miRNA expression by next-generation sequencing with a two-step normalization of miRNA sequence data. The miRNAs involved in IPMN associated with invasive carcinoma were identified from this tissue analysis and further verified with the pancreatic juice samples. From the tumor-normal paired tissue analysis of the expression levels of 2792 miRNAs, 20 upregulated and 17 downregulated miRNAs were identified. In IPMN associated with invasive carcinoma (INV), miR-10a-5p and miR-221-3p were upregulated and miR-148a-3p was downregulated when compared with noninvasive IPMN. When these findings were further validated with pancreatic juice samples, miR-10a-5p was found to be elevated in INV (p = 0.002). Therefore, three differentially expressed miRNAs were identified in tissues with INV, and the expression of miR-10a-5p was also elevated in pancreatic juice samples with INV. MiR-10a-5p is a promising additional biomarker for invasive IPMN.     

铝合金局部加压凝固及流动数值模拟的研究

局部加压方法是压铸中用于消除铸件厚壁处缩孔缺陷的一种新工艺.本研究通过实验模型测量了不同加压条件下铸件的冷却曲线,分析了局部加压引起的凝固变化.考虑局部加压引起的补缩流动,通过适时修改网格文件、初始和边界条件的方法,模拟了局部加压过程中铝合金的凝固及流动变化,并与实验结果进行了对比,二者基本吻合.     

Characterization of New DNA Aptamers for Anti-HIV-1 Reverse Transcriptase

HIV-1 RT is a necessary enzyme for retroviral replication, which is the main target for antiviral therapy against AIDS. Effective anti-HIV-1 RT drugs are divided into two groups; nucleoside inhibitors (NRTI) and non-nucleoside inhibitors (NNRTI), which inhibit DNA polymerase. In this study, new DNA aptamers were isolated as anti-HIV-1 RT inhibitors. The selected DNA aptamer (WT62) presented with high affinity and inhibition against wild-type (WT) HIV-1 RT and gave a KD value of 75.10±0.29 nM and an IC₅₀ value of 84.81±8.54 nM. Moreover, WT62 decreased the DNA polymerase function of K103 N/Y181 C double mutant (KY) HIV-1 RT by around 80 %. Furthermore, the ITC results showed that this aptamer has small binding enthalpies with both WT and KY HIV-1 RTs through which the complex might form a hydrophobic interaction or noncovalent bonding. The NMR result also suggested that the WT62 aptamer could bind with both WT and KY mutant HIV-1 RTs at the connection domain.     

Formation of Small-Sized CdSxSe1-x Crystals in Sol-Gel-Derived SiO2 Glasses

Silica glasses doped with small-sized CdS_x-Se_1-x crystals were prepared by the sol-gel method. Gels synthesized by the hydrolysis of Si(OC₂H₅)₄, in the presence of CdSeO₄ with NH₄SCN dissolved in HNO₃ or NH₄OH, were heated in H₂-N₂ atmosphere. The pH value of solutions for CdSeO⁴ and NH⁴SCN primarily determined the fraction of anions in CdS^xSe^1-x crystals. The anion content in crystals was dependent on the pH value of the solvent and/or heat-treatment temperature, and the sulfur fraction changed from 0.1 to 0.6. The optical absorption spectra were red-shifted as the selenium content and the crystal size increased, and the emission spectra showed a sharp band near the absorption edge position and a broad tail extending into the long wavelength. The optical band gap energies increased reciprocally proportional to the square of the crystal size.     

Effect of Silicon Dioxide on the Thermal Diffusivity of Aluminum Nitride Ceramics

The thermal diffusivity of AlN ceramics was significantly decreased by the addition of SiO₂. The AlN ceramics with 4 wt% SiO₂ could not be densified by pressureless sintering in the temperature range 1400° to 1800°C. The thermal diffusivity of these samples was very low because of their porous structure. The AlN ceramics containing 2, 4, and 8 wt% SiO₂ were densified by hot-pressing and also had low thermal diffusivity. In these samples, the grains of the 27R polytype that resulted from the reaction between AlN and SiO₂ were dispersed, obstructing the conduction of heat. The relation between the amount of 27R polytype and the thermal diffusivity of the AlN ceramics was determined.     

A thermomechanical investigation of highly cold-drawn polycarbonate

A new technique detecting molecular motions in drawn polymers was applied to highly cold-drawn polycarbonate of bisphenol A. It is shown that the sample exhibits thermal shrinkage in three steps with the temperature increase up to above the glass transition temperature. The molecular relaxation at the highest temperature is due to the glass transition. The other two molecular motions at the lower temperature are those of main chain in the glassy state and they correspond to the molecular motions as revealed in dielectric measurement by Sacher.¹ By using the general theory of the thermal analysis by Ozawa,² the apparent activation energies of these molecular motions were obtained: for the highest temperature 110 kcal/mole, and for the lowest temperature, 33.5 kcal/mole. The impact strength and the cold workability of this polymer are also discussed in relation to these molecular motions.     

Fractal characteristics of gas-solids flow in a circulating fluidized bed

A fractal approach is adopted to describe the dynamic behavior of a circulating fluidized bed. Two times series, differential pressure fluctuations along the riser height and solids momentum fluctuations along the radial direction, are measured and analyzed in terms of fractal dimensions. The influences of operating conditions and axial/radial positions on the fractal dimension are discussed. Attempts are also made to interpret the flow structure in the bed in terms of the fractal dimension. It is found that fractal analysis can provide a useful tool for understanding the characteristics of gas-solids flow in circulating fluidized beds.     

Synthesis of poly[(methyl methacrylate)-co-acrylamide] modified by titanium-triisopropoxide and their thermal stability

Poly[(methyl methacrylate)-co-acrylamide] of various compositions were previously prepared by the radical copolymerization reaction of methyl methacrylate and the acrylamide monomer in a solvent. The copolymers were modified through a resonating salt by the addition reaction of a chlorotitanium-triisopropoxide; the modified resins are called Ti hybrid copolymers. The chemical structure of acrylamide side chain groups in the Ti hybrid copolymers was confirmed by the ash weight after combustion, the characteristic absorption band determined by Fourier transform infrared spectrophotometry (IR) and elementary analysis (EA). The curing temperature and time of the resins were determined from the change in the insoluble resin weight when the extraction was done with a mixture of acetone and methanol using the curing resin samples. The thermal stability of the Ti hybrid copolymers was evaluated using both the thermal-degradation temperature (T_x) measured by thermogravimetric analysis and the thermal degradation at 130 °C for 720 h. The cured resins improved the weight loss of the resins at the T_x, and exhibited a high thermal stability.     

Formation of 2,4-dinitrophenylhydrazones in high-density polyethylene film irradiated with ultraviolet light

Polyethylene films were irradiated in air by ultraviolet light. 2,4-Dinitrophenylhydrazine was reacted on the irradiated films. The changes in amounts of carbonyl groups and 2,4-dinitrophenynlhydrazones formed in the films were inferred by comparing their absorptions in the infrared and ultraviolet spectra, respectively. It seems that the contents of carbonyl groups formed in the amorphous regions in the high-density polyethylene films by the irradiation were larger than the contents of carbonyl groups formed in the amorphous regions in the low-density polyethylene films. The decreases in contact angles of water on the high-density polyethylene films by the irradiation were larger than the decreases in the contact angles on the low-density polyethylene films. The amounts of 2,4-dinitrophenylhydrazones formed in the irradiated high-density polyethylene films were less than the amounts of 2,4-dinitrophenylhydrazones formed in the irradiated low-density polyethylene films.     

Feeding-stimulant activity of algal glycerolipids for marine herbivorous gastropods

Phagostimulant activity of glycerolipids such as digalactosyldiacylglycerol (DGDG), 6-sulfoquinovosyldiacylglycerol (SQDG), phosphatidylcholine (PC), phosphatidylethanolamine (PE), etc., have been examined using the Avicel plate method for three kinds of marine herbivorous gastropods, the abaloneHaliotis discus, the turban shellTurbo cornutus, and the topshellOmphalius pfeifferi. DGDG showed strong activity for all the test animals. SQDG was much less active than the other glycerolipids for abalone. The turban shell and the top shell responded more or less sensitively to all tested glycerolipids at doses of 10–20g/sample zone.Chemical Studies on Phagostimulants for Marine Gastropods. Part VIII. For Part VII, see Sakata et al. (1988a).